In addition, the lab is equipped with inverted and upright microscopes, a digitized fluorescence microscopy station, a fluorescence activated cell sorter, an autoclave and cryopreservation facilities. A confocal microscope, housed in the basement of the building is also available for experimental use. The cell and tissue engineering laboratory is thus suitable for mammalian tissue culture, cell function analysis, cell separation and protein expression evaluation. The cell and tissue analysis and characterization lab contains a full complement of biochemical and molecular biological equipment including vertical and horizontal gel boxes, power supplies, a spectrophotometer, a gel imaging and analysis workstation, several microcentrifuges, a -80°C freezer and all necessary biochemicals and electrophoresis supplies needed for protein and nucleic acid separation and analysis. This facility also contains 3 biosafety cabinets and a light cycler for nucleic acid isolation, purification, amplification and general PCR technology. In addition, a separate bacterial culture and analysis laboratory contains a bacterial culture incubator and independent centrifuges and electrophoresis gel boxes. These laboratories are thus equipped for dynamic evaluation of cells and tissues from the macroscopic and microscopic levels to molecular analysis of protein and gene expression.
BioMEMS and Microfabrication Laboratory (Zahn)
This laboratory integrates various engineering disciplines with biological and medical applications, creating a highly technical and thoroughly collaborative biomedical engineering environment. It maintains a microfabrication facility housed in BME building in a 1000square feet clean room with photolithography, PVD, and other equipment. For further details, please visit the BioMEMS site.
Stem Cell Biology and Bioengineering Laboratory (Cai)
The laboratory is equipped for all surgical, histological, molecular recombinant DNA techniques, with dedicated microscope room for imaging analysis.
Animal: Mouse (protocol # 05:060) and Chick Embryos. A modern animal facility is available in the basement of the BME Building and Nelson Biological Laboratories building. Investigators pay a per diem charge. It is equipped with bottle and cage washers as well as surgical areas and a bio-safety cabinet.
Computer: Office and laboratory computers includes 10 high-end PCs for computational biology and data analyses, 2 Linux-based lab servers (cell.rutgers.edu and temcell.rutgers.edu) for web-based applications, genome sequence and gene expression data analysis, data storage and dissemination; one backup server to secure data on all office and lab computers, including lab servers. All computers are linked via the university LAN, NCBI, PubMed, and to the lab servers. The lab servers have a total capacity of over 3000 GB disk space. The non-coding DNA sequence retrieval system (NCSRS) developed in house is on “cell.rutgers.edu/ncsrs”. Data is managed with database systems (MySQL and PostgreSQL). Data analysis software includes Vector NTI, MatLab, Adobe Photoshop, Zeiss AxioVision, Microsoft Office, etc. Laser printers and scanners meet personal and group computer needs.
The laboratory is fully equipped for developmental biology, neuroscience, molecular biology, and computational biology. Major equipment items in the PI’s laboratory include:
• PCR machine (MJ Research PTC-200) for recombinant DNA applications
• electroporator (BTX ECM 830) for in vivo/ovo electroporation applications
• cryostat (Thermo 620E) for tissue sectioning
• fluorescence stereomicroscope (Leica M16FA)
• injection station for mouse and chick embryos
• epifluorescence upright microscope (Zeiss Axio Imager M1) for image data analysis
• ultralow (- 80 °C) freezer, regular freezer and refrigerator, etc.
Major equipment items within the Department of Biomedical Engineering include:
• high-speed and ultracentrifuges
• bio-safety and laminar-flow hoods
• bacterial incubators/shakers, gamma counters
• small animal facility
The Biomechanics and Rehabilitation Engineering Laboratory (Craelius)
This laboratory is located in the Engineering Building on Rutgers University's Busch Campus. The BREL lab is specifically interested in topics of neuro-rehabilitation and motor control. Major projects within the lab investigate novel tools for the rehabilitation of chronic stroke, cerebral palsy, and spinal cord injury victims, as well as characterization of aspects of manual and pedal motor control in normal and afflicted subjects.
BME maintains privileges at the Keck Center for Collaborative Neuroscience, which includes a microarrayer for gene chip analysis and a confocal microscope. In addition, privileges are maintained at the RWJMS Core Facility for nucleic acid synthesis and at EOSHI on the Rutgers campus, for high speed cell sorter and analysis.